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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: Am J Reprod Immunol. 2011 Jan 18;65(6):597–609. doi: 10.1111/j.1600-0897.2010.00968.x

Fig. 5.

Fig. 5

Differential expression of CD8+ T cell activation markers following co-culture with NPL-loaded DC. 72 hrs post-exposure to NPL DC or control Ag forms, CD8+ cells exposed to NPL-loaded DC express markers of positive activation, such as ICOS, while failing to up-regulate negative co-stimulators such as PD-1 and CTLA-4 (panel four). Conversely, CD8+ cells exposed to lysate-loaded DC express increased cell surface inhibitory molecules such as PD-1 and CTLA-4, but comparably little ICOS (panel three). Positive control CD8+ T cells stimulated non-specifically with PMA/ionomycin show strongly increased expression of all markers (panel five), while control unloaded DC (panel one) and DC loaded with empty NP (panel two) show little or no up-regulation [DC +CD8= unloaded DC in co-culture, DC NP +CD8= DC loaded with empty NP, DC L + CD8= soluble lysate-loaded DC, DC NPL +CD8= NPL-loaded DC, CD8 + PMA= T cells non-specifically stimulated with PMA/ionomycin].