AKBA suppresses invasion in pancreatic cancer cells. A, PANC-28 cells (3 × 104 cells/well) were seeded in the top chamber of a Matrigel. After preincubation with or without AKBA (50 μmol/L) for 6 hours, the transwell chambers were placed into 24-well plates with basal medium only or basal medium containing 100 ng/mL CXCL12. After incubation, the cell invasion assay was done as described in Materials and Methods. B, Columns mean number of invaded cells, bars SE. *P<0.01