FIGURE 3. IL-2 complexes preferentially inhibit myelin-reactive Th17 cells in the CNS.

Mice were primed with PLP/CFA and treated with IgG or IL-2 complexes (IL-2 C). Mice were sacrificed at day 10–25 post-immunization (p.i.). Lymph node and CNS cells were isolated. (A) Expansion of CD4⁺ T cells in the periphery and in the CNS on gated lymphocytes was assessed by CFSE dilution or BrdU incorporation assay, respectively. (B) Lymphoid or CNS cells were re-stimulated with PLP overnight and IFN-γ- and IL-17-expressing CD4⁺ T cells were measured by intracellular staining. FACS dot plots are representative of four independent experiments (n= 6–15/group). Bar data were accumulated from four independent experiments; P values were calculated by Student’s t-test.