Fig. 7.

a GFP-Trap experiments. Lysates from L. donovani wild-type or L. donovani [pTL-LdSGT::eGFP] cells were bound to GFP-Trap-A resin and eluted. Fractions (I input, FT flow-through, B bound) were subjected to SDS-PAGE and Western blotting with mouse antisera against selected chaperones and co-chaperones as indicated to the right. Note that staining intensity does not necessarily reflect quantities due to different development parameters. b Schematic drawing of SGT-containing, mammalian foldosome complex redrawn from Buchanan et al. (2007)