Fig. 5.

Expression of Hsp90 in the foot, kidney, and hepatopancreas tissues of S. cariosa and S. zonata. Total protein was extracted from fully hydrated control and desiccated snails during 10 days of desiccation and subjected to Western blotting. A representative blot of three independent experiments in the foot (a) and kidney (b) is shown. Arrows denote the point of connection where two consecutive gels were combined. Relative levels of Hsp90 in the foot (c), kidney (d), and hepatopancreas (e) are shown. n = 6–15 snails. Different letters above the bars denote significant differences within each species (lower case—S. cariosa; upper case—S. zonata). Asterisks denote significant differences between the two species (*p < 0.05; **p < 0.01; ***p < 0.001)