Table 1.

Correlation between sperm vacuoles and DNA fragmentation in spermatozoa

Sperm sample	Number sperm analysed	TUNEL positive/MSOME normal (%)	TUNEL positive/MSOME abnormal (%)	Significance (p-value)
1	102	1/64 (1.5%)	6/38 (15.8%)	0.02
2	114	0/37 (0%)	12/77 (15.6%)	0.03
3	86	3/16 (18.8%)	3/70 (4.36%)	0.86
4	100	5/31 (16.1%)	4/69 (5.8%)	0.80
5	158	1/75 (1.3%)	9/83 (10.8%)	0.04
6	88	1/34 (2.9%)	12/54 (22.2%)	0.03
7	112	2/41 (4.9%)	16/71 (22.5%)	0.03
8	100	1/33 (3.0%)	14/67 (20.9%)	0.04

Spermatozoa from 8 diverse semen samples were analysed and separated as MSOME normal and abnormal. TUNEL assay was performed on these same spermatozoa. The number of TUNEL assay positive (i.e. with fragmented DNA) spermatozoa is shown in each sample. Proportions are shown in parentheses. The two-tailed z-test was used to calculate the significance, p < 0.05 is considered significant