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. Author manuscript; available in PMC: 2012 Apr 8.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Mar 5;407(2):301–306. doi: 10.1016/j.bbrc.2011.03.006

Fig. 2. Soluble CD4-modified AFM tips detected low-abundant gp120 proteins mixed with highly concentrated monkey serum proteins.

Fig. 2

(a) Interaction force histogram obtained in PBS between sCD4 tips and the mixture of 0.01 mg/ml (low-abundant) gp120 and 1 mg/ml (high-abundant) serum. The histogram obtained from 1243 force-distance curves (n=1243) reveals a distribution of interaction forces with a generalized extreme value at 21.59 ± 20.19 pN (mean value: 39.11± 35.78 pN). The arrow indicates the curves without interaction force (~7.5%). (b) Interaction force histogram (n=1000) obtained in PBS using the same molecule pairs under the same condition as Fig. 2a, except that the gp120 proteins were denatured by heat at 100°C for 3 min prior to the mixing of gp120 with serum. Generalized extreme value: 25.56 ± 34.66 pN; mean value: 49.88 ± 49.78 pN. The arrow indicates the curves without interaction force (~26.2%). (c) Interaction force histogram (n=1000) between sCD4 tips and BSA (1 mg/ml). The arrow indicates the curves without interaction force (~83.0%).