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GP image analysis. a Laurdan GP image (equatorial z-section) of a GUV made of DOPC/DPPC/FC 1:1:1 at 25°C showing phase separation, l o (orange) and l d (green). The SimFCS program was used to analyze the GP image. Pixels with different GP values can be separated and the individual l o (b) and l d (c) phases followed in time. The number of pixels is related to the area of each phase (Color figure online)
