Figure 2.

Gastric cancer cell supernatant mediates the conversion of CD4⁺CD25^- T cell to CD4⁺Foxp3⁺ Tregs. A: Representative flow cytometry analysis of CD4+Foxp3+ Tregs frequency in CD4⁺ T cells population following sorted CD4⁺CD25^-CD127⁺ T cells co-culture with medium, GES, or MGC supernatants; B: Summarized data showed that both MGC and SGC supernatants induced higher CD4⁺Foxp3⁺ Tregs (^aP < 0.05); C: After co-culture with MGC, GES or medium, CD4⁺CD25^- T cells were placed in coculture with CFSE-labeled CD4⁺CD25^-CD127⁺ T cells at a ratio of 1:1 in the presence of soluble anti-CD3/CD28 as well as IL-2. The representative data from three independent experiments are shown.