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. 2011 Mar 26;6(2):64–68. doi: 10.6026/97320630006064

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© 2011 Biomedical Informatics

This is an open-access article, which permits unrestricted use, distribution, and reproduction in any medium, for non-commercial purposes, provided the original author and source are credited.

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A. Multiple sequence alignments of ITS2 sequence and flanking regions of Fasciolopsis buski, Fasciola gigantica and Paragonimus westermani; B. Pattern matching of ITS motifs of different geographical isolates of fasciolid species: Fasciolopsis buski India (481bp), Paragonimus westermani India (496bp), Fasciola gigantica India (606bp); C. Predicted ITS2 RNA secondary structures and their structure formation enthalpies according to MFOLD; D. Results of PCR amplification of DNA (M= 100-bp DNA ladder marker, C= Control primers 3S-A28); E. Phylogenetic relationships between members of family Paragonimidae and Fasciolidae. The Tree shows hypothetical Bayesian analysis phylogeny based on the secondary structure alignment data of the ITS2 region. The numbers are equivalent to bootstrap percentages.