Skip to main content
. 2010 Dec 11;39(8):3363–3372. doi: 10.1093/nar/gkq946

Table 2.

Determination of Tm values of different neomycin-binding aptamers

Aptamer Tm in °Ca 0 µM neomycin Tm in °Ca 10 µM neomycin ΔTm Regulatory Factorb
N1-3/0-CUU 50.5 ± 0.2 71.8 ± 0.5 21.3 3.9
R23 57.5 ± 0.5 73.1 ± 0.6 15.6 1.1
N1(R23) 51.3 ± 0.5 71.5 ± 0.4 20.2 1.6
N1(A) 54.6 ± 0.5 68.3 ± 0.8 13.7 1.0
N1-3/0-CUC 50.5 ± 0.2 72.5 ± 0.3 22.0 3.5
N1-2/0-CU n.d. 74.0 ± 0.4 n.d. 3.7
N1-1/0-C 61.0 ± 0.5 77.1 ± 0.2 16.1 1.9
N1-1/0-U 61.6 ± 0.5 77.6 ± 0.7 16.1 1.8
N1-0/0 74.2 ± 0.4 80.5 ± 0.5 6.3 1.0
N1-2/1-CU/A 60.3 ± 0.3 71.4 ± 0.5 11.1 1.1
N1-3/1-CUU/A 56.0 ± 0.5 69.3 ± 0.8 13.3 1.1

n.d.: not determined. The aptamer N1-2/0-CU shows biphasic melting behavior in the absence of neomycin.

aMelting analysis was performed using 1 µM aptamer RNA in 20 mM Na-cacodylate pH 6.8 and 100 mM NaCl. Melting curves were recorded in triplicates. Buffer with or without neomycin was analyzed in parallel as a blank and substracted from all data.

bEfficiency of regulation is given as the ratio of relative fluorescence with and without neomycin.