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. 2010 Dec 11;39(8):3188–3203. doi: 10.1093/nar/gkq1242

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Transcriptional analysis of the nirB mRNA. (A) Microarray data for nirB and adjacent genes. Data are presented as described in Figure 1. (B) Primer extension analysis of the nirB transcript. The primer extension was performed as described in the ‘Materials and Methods’ section. The transcription start site of the nirB operon transcript is indicated by an open circle. The diamond denotes a cleavage site found in all three strains located 2 nt upstream of the AUG translation start site, while the open triangle marks the start of the translation start codon in the DNA sequencing ladder. The asterisk (bottom right) indicates a major 5′ terminus, located within the nirB coding sequence that is not seen in the absence of RNase III. The putative RNase III cleavage site (in bold/underlined) is shown in the expanded sequence on the bottom left of the figure.