Table 1.
Comparison of array data with published experiments that employed various RNase E mutants
| Gene/operon | Published observation in absence of RNase E | References | Observed change in steady-state RNA levels on array |
|---|---|---|---|
| rpsT | Increased half-life | (22,58) | Increased abundance of transcript |
| rpsO | Increased half-life | (13,22) | Increased abundance of transcript |
| cspE | Increased half-life | (58) | Increased abundance of transcript |
| htpG | Increased half-life | (58) | Increased abundance of transcript |
| glpQ | Increased half-life | (58) | Increased abundance of transcript |
| raiA | No change in half-life | (22) | No change in transcript abundance |
| trxA | No change in half-life | (22) | No change in transcript abundance |
| csrC | Increased half-life | (84) | Increased abundance of transcript |
| rrl rrs | Increased stability of spacer region between 23S and 5S sequences | (22) | Increased abundance of spacer region between mature 23S and 5S rRNA sequences |
| pheU | Increased abundance of full-length tRNA precursor | (3) | Increased abundance of terminator region |
| secG leuU | Increased half-life of secG mRNA and stabilization of leuU terminator region | (5) | Increased abundance of secG mRNA and leuU terminator region |
| glyW cysT leuZ | Increased abundance of polycistronic transcript | (3,19) | Increased abundance of spacer regions |
| tyrT tyrV tpr | Increased abundance of polycistronic transcript | (3,19) | Increased abundance of spacer regions |
| metT leuW glnU glnW metU glnV glnX | Increased abundance of polycistronic transcript | (5) | Increased abundance of spacer regions |
| argX hisR leuT proM | Increased abundance of polycistronic transcript | (3,19) | Increased abundance of spacer regions |
| valV valW | No change in abundance of polycistronic transcript | (4) | No change in abundance of polycistronic transcript |
| leuQ leuP leuV | No change in abundance of polycistronic transcript | (4) | No change in abundance of polycistronic transcript |
| metV metW metZ | No change in abundance of polycistronic transcript | (4) | No change in abundance of polycistronic transcript |
In the case of mRNAs, published data relates to observed changes in individual half-lives. For tRNA transcripts, the published data relate to whether inactivation of RNase E led to changes in the levels of the full-length mono- or polycistronic transcripts.