Table 2.
Gene | Mutant/wild type | Fold change from array | Fold change from northern analysis |
---|---|---|---|
cspE | ΔRNase E | 4.0 | 3.4 |
ΔRNase III | 2.2 | 1.3 | |
dnaK | ΔRNase E | 2.0 | 2.3 |
ΔRNase III | 2.0 | 1.3 | |
ompF | ΔRNase E | −1.6 | −1.3 |
ΔRNase III | 2.0 | 2.7 | |
ryhB | ΔRNase E | 7.6 | 20.4 |
ΔRNase III | 5.4 | 3.7 | |
ssrS | ΔRNase E | ND | −1.6 |
ΔRNase III | ND | 1.1 |
Positive values indicate higher abundance in the mutant, while negative values indicate higher abundance in wild type. Fold changes from the array data were calculated by averaging the ratios of every oligonucleotide probe across the genomic feature (see ‘Materials and Methods’ section). Fold change numbers from the northern analyses were calculated as described in the ‘Materials and Methods’ section. ND, not determined due to the nature of the abundance change of ssrS (see Supplementary Figure S6).