Table 2.
Tomato plant and Paramecium variant telomeric sequences and Tms determined by analysis of melting curves recorded at 295 nm, at 3 µM oligonucleotide strand concentration
Sequence name | Sequence (from 5′ to 3′) | Tm (°C) in NaCl | Tm (°C) in KCl |
---|---|---|---|
Tomato plant | |||
Tom24 | G3TTAAG3TTAAG3TTAAG3 | 55 | 67 |
TAA | G3TTTAG3TTAAG3TTAAG3 | 58 | 65 |
ATA | G3TTAAG3TTTAG3TTAAG3 | 57 | 69 |
AAT | G3TTAAG3TTAAG3TTTAG3 | 58 | 67 |
TTA | G3TTTAG3TTTAG3TTAAG3 | 58 | 66 |
TAT | G3TTTAG3TTAAG3TTTAG3 | 57 | 64 |
ATT | G3TTAAG3TTTAG3TTTAG3 | 56 | 67 |
Ara24 | G3TTTAG3TTTAG3TTTAG3 | 58 | 66 |
Paramecium | |||
Par21 | G3TTTG3TTTG3TTTG3 | 52 | 68 |
GTT | G3GTTG3TTTG3TTTG3 | 54 | 72 |
TGT | G3TTTG3GTTG3TTTG3 | 52 | 68 |
TTG | G3TTTG3TTTG3GTTG3 | 59 | 72 |
GGT | G3GTTG3GTTG3TTTG3 | 54 | 71 |
GTG | G3GTTG3TTTG3GTTG3 | 58 | 74 |
TGG | G3TTTG3GTTG3GTTG3 | 58 | 73 |
GGG | G3GTTG3GTTG3GTTG3 | 60 | 76 |
Tms reported in this table were obtained on a different spectrophotometer from those reported in Table 1.
Temperature was measured with a sensor in a sealed cuvet provided by the manufacturer; this may explain the slightly higher Tms reported in this table as compared to Tms reported in Table 1 for Tom24, Ara24 and Par21.
TDS and CD spectra are reported in Supplementary Figures S4 and S5.