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. 2010 Dec 21;39(8):3282–3294. doi: 10.1093/nar/gkq1292

Table 2.

Tomato plant and Paramecium variant telomeric sequences and Tms determined by analysis of melting curves recorded at 295 nm, at 3 µM oligonucleotide strand concentration

Sequence name Sequence (from 5′ to 3′) Tm (°C) in NaCl Tm (°C) in KCl
Tomato plant
    Tom24 G3TTAAG3TTAAG3TTAAG3 55 67
    TAA G3TTTAG3TTAAG3TTAAG3 58 65
    ATA G3TTAAG3TTTAG3TTAAG3 57 69
    AAT G3TTAAG3TTAAG3TTTAG3 58 67
    TTA G3TTTAG3TTTAG3TTAAG3 58 66
    TAT G3TTTAG3TTAAG3TTTAG3 57 64
    ATT G3TTAAG3TTTAG3TTTAG3 56 67
    Ara24 G3TTTAG3TTTAG3TTTAG3 58 66
Paramecium
    Par21 G3TTTG3TTTG3TTTG3 52 68
    GTT G3GTTG3TTTG3TTTG3 54 72
    TGT G3TTTG3GTTG3TTTG3 52 68
    TTG G3TTTG3TTTG3GTTG3 59 72
    GGT G3GTTG3GTTG3TTTG3 54 71
    GTG G3GTTG3TTTG3GTTG3 58 74
    TGG G3TTTG3GTTG3GTTG3 58 73
    GGG G3GTTG3GTTG3GTTG3 60 76

Tms reported in this table were obtained on a different spectrophotometer from those reported in Table 1.

Temperature was measured with a sensor in a sealed cuvet provided by the manufacturer; this may explain the slightly higher Tms reported in this table as compared to Tms reported in Table 1 for Tom24, Ara24 and Par21.

TDS and CD spectra are reported in Supplementary Figures S4 and S5.

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