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. 2010 Dec 21;39(8):3079–3092. doi: 10.1093/nar/gkq1272

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of pheL and pdxH 5′ and 3′ sequences on frameshifting at CCC_UGA. (A) Scheme of the analysed constructs. (B) SDS–PAGE of proteins expressed from pheL, pdxH, HLLH, HLH5 and HLH3 constructs. The termination, GST–PheL, and the frameshift, GST–PheL–MBP, products are indicated by arrows. (C) Quantitation of frameshifting efficiency during translation of pheL, pdxH, HLLH, HLH5 and HLH3 constructs from (B). The level of frameshifting obtained with the WT pheL sequence is set to 100% of wild-type.