Figure 1.

Messenger RNA for polb is plentiful in early stage zebrafish embryos. Developmental stages are indicated as hours post-fertilization (hpf). Values represent the average of 3 independent experiments ± SD of the mean. (A) Northern blot of total RNA extracted from different stage embryos and adults. Each lane was loaded with 30 µg total RNA and probed with α[³²P] dCTP labeled polb cDNA. (B) qRT–PCR quantitation of polb mRNA expression within the first 24 h of development. Data were generated from two independent experiments.