Figure 5.

Apex regulates proteins in the Creb-dependent transcription complex. (A) Transcripts of proteins that participate in the Creb complex were measured by qRT–PCR in control and Apex knockdown 24 hpf embryos. Messenger RNA for Crtc1, Crtc3, Cbp and Crem were examined using primers described in Supplementary Table S1. Data are presented as ratio of control normalized for b-actin message. Values are the average of three independent experiments ± SD of the mean. (B) Western blot analysis showed that proteins in the Creb complex (Creb1 itself, Crtc1, Cbp and Crem) are decreased when Apex is knocked down. We did not quantify Crtc3, as there is no commercially available antiserum. (C) Quantitation of western blot results of Creb complex proteins in 24 hpf control and Apex knockdown embryos. Values are the average of three independent experiments ± SD of the mean.