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. 2011 Feb 3;39(8):e50. doi: 10.1093/nar/gkr034

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Effect of Hfq on atsRNA-mediated gene silencing. (A) The effect of atsRNA GY1, GY2 and GY6 on EGFP gene in strains MC4100 and its hfq mutant GSO81. (B) The effect of atsRNA CY4, CY6 and CY9 on uidA gene in strains MC4100 and its hfq mutant GSO81. (C) The stability of atsRNA GY2 in MC4100 and its hfq mutant GSO81. (D) The stability of atsRNA CY9 in MC4100 and its hfq mutant GSO81. In (A) and (B), bacteria cells containing empty vector pRI were used as the control. The expression of target gene was measured after the induction of atsRNA with 1 mM IPTG for 30 min. Average values were from triplicate experiments with standard values indicated. In (C) and (D), the expression of atsRNAs was induced with 1 mM IPTG for 30 min, and then rifampicin was added. The incubation was continued further and cells were harvested at the indicated time points for RNA preparation. Total RNA was analyzed using probes specific for GY2, CY9 and 5S rRNA, respectively.