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. 2011 Feb 3;39(8):e50. doi: 10.1093/nar/gkr034

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Effect of atsRNAs targeting against essential genes on growth rate of host bacteria. Growth curves of MC4100 strains after the expression of atsRNAs targeting against murA (A), trmA (B) and ygjD (C). Overnight cultures were diluted 1:100 into LB medium and bacterial strains were grown with 0.1 mM IPTG at time 0 at 30°C. (D) Growth curve of MC4100 strain after the expression of atsRNAs M1, T1 and Y1. Bacterial cells containing empty vector pRI and atsRNA CY2 were used as the control. Turbidity of the cultures was monitored at 600 nm.