Table 2.
UV melting | van't Hoff analysis of UV melting curves |
UV melting from δα/δ (tM−1) | UV melting, concentration dependence |
DSC | ||||
---|---|---|---|---|---|---|---|---|
Column #1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
Oligonucleotide (abbreviation) | Tm (°C) | −ΔH (kcalmol−1) | −ΔS (eu) | −ΔG (kcalmol−1) | −ΔH (kcalmol−1) | −ΔH (kcalmol−1) | −ΔS (eu) | −ΔH (kcalmol−1) |
5′-r(CGAAUUCG)-3′ (rA2U2) | 34.1 ± 0.6 | 63.4 ± 6.6 | 180.0 ± 21.6 | 7.5 ± 0.2 | 55.6 ± 4.6 | 58.0 ± 9.4 | 189.7 ± 30.6 | 39.2 ± 1.4 |
5′-fCrGfArAfUrUfCrG-3′ (f/rA2U2) | 43.7 ± 0.4 | 63.8 ± 3.9 | 175.2 ± 12.0 | 9.4 ± 0.2 | 58.4 ± 3.7 | 58.3 ± 12.9 | 185.8 ± 41.0 | 48.8 ± 0.3 |
5′-f(CGAAUUCG)-3′ (fA2U2) | 53.3 ± 0.3 | 69.5 ± 2.0 | 186.9 ± 6.2 | 11.6 ± 0.1 | 62.1 ± 2.9 | 62.3 ± 9.8 | 192.1 ± 30.2 | 53.5 ± 0.7 |
5′-r(UAUAUAUAUAUA)-3′ (rUA6) | 31.7 ± 0.4 | 70.9 ± 5.9 | 206.5 ± 19.1 | 6.9 ± 0.1 | 66.0 ± 2.1 | 82.5 ± 7.8 | 271.6 ± 25.8 | 49.5 ± 0.5 |
5′-fUrAfUrAfUrAfUrAfUrAfUrA-3′ (f/rUA6) | 44.1 ± 0.4 | 83.5 ± 4.8 | 237.2 ± 15.1 | 10.0 ± 0.1 | 78.4 ± 5.1 | 75.9 ± 9.5 | 241.5 ± 30.4 | 54.3 ± 0.4 |
5′-f(UAUAUAUAUAUA)-3′ (fUA6) | 53.1 ± 0.5 | 83.7 ± 4.1 | 230.8 ± 12.6 | 12.2 ± 0.2 | 80.2 ± 5.4 | 79.1 ± 14.0 | 244.7 ± 43.4 | 56.6 ± 0.7 |