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. 2010 Dec 21;39(8):3053–3063. doi: 10.1093/nar/gkq1209

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — p53 binding to the –2242 bp RE is affected by HATi Garcinol. (A) Comparison of p53 binding activities on p21 following UVB irradiation. p53 binding to the –1354, –2242 and –11 708 bp REs was measured by DLF in wt fibroblasts exposed to 500 and 2000 J/m² UVB. (B) Effect of Garcinol on p53 protein levels in wt fibroblasts exposed to UVB. p53 levels were measured by western blot. (C) Effect of Garcinol on p53 binding in vitro on naked DNA following UVB irradiation. p53 binding to the –2242 bp RE was measured by MAPD. (D) Effect of Garcinol on p53 binding in cells on chromatin following UVB irradiation. p53 binding to the –2242 bp RE was measured in wt fibroblasts using DLF. The gel obtained using an automated sequencer is presented, negative footprints are indicated by a bar. (E) Quantification of negative footprint intensity measured by DLF at the –2242 bp RE. (F) Effect of Garcinol on histone H3 and histone H4 acetylation following UVB irradiation. Levels of histone H3, acetylated histone H3 (H3Ac), and acetylated histone H4 (H4Ac) were measured by ChIP at the –2242 and –3969 bp REs. Results are expressed as ratios of acetylated histone on histone H3.