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. 2010 Dec 30;39(8):3176–3187. doi: 10.1093/nar/gkq1318

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Circadian oscillation of the acetylation of XPA. (A) Protein levels in liver cytosol and nuclear extracts were analyzed by immunoblotting with the indicated antibodies including histone H3 (H3). (B) Acetylated proteins from mouse liver cytosol and nuclear extracts were immunoprecipitated using acetyl-lysine antibody and the level of XPA was analyzed by immunoblotting for XPA. (C) Quantitative analysis of the acetylated XPA in the liver nuclear extract. Average values and standard deviations are shown from two independent experiments with two technical measurements on each together with XPA protein expression and DNA excision repair activity data from a previous report (14). (D) Acetylated proteins from mouse liver nuclear extracts were immunoprecipitated with anti-acetyl-lysine antibodies (1° IP). The immunodepleted-lysate from 1° IP was used for second round of immunoprecipitation (2° IP). The level of acetylated XPA from each IP was analyzed and compared with the input signal (10% of nuclear extracts used for IP) by immunoblotting for XPA.