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. 2010 Dec 21;39(8):3224–3239. doi: 10.1093/nar/gkq1289

Figure 7.

Figure 7.

Silencing of the Arxes in C3H10T1/2 pluripotent stem cells reduces adipogenesis and enhances osteoblastogenesis. C3H10T1/2 cells were transduced with silencing constructs (siArxes) or nontargeting control (ntc), and induced to undergo adipogenesis or osteoblastogenesis for 10 days as described in ‘Material and Methods’. Results are representative of two independent replicates each performed in triplicates (mean ± SEM). (A) Expression of PPARγ was measured with qPCR before differentiation and on Day 10 of adipogenesis in Arxes-silenced and control cells. Arxes CDS primer pair recognizes mRNA levels of both Arxes as it is aimed at the coding sequence. (B) Oil red O staining shows reduced lipid droplet formation in Arxes-silenced cells at Day 10 of adipogenesis, which is in concordance to the results in 3T3-L1 cells. (C) qPCR measurements show that mRNA levels of ALP are increased in Arxes-silenced cells. (D) At Day 10 of osteoblastogenesis cells were homogenized and mixed with pNPP solution and kinetics were recorded for 3 min using a spectrophotometer at 405 nm. Measurements were normalized to protein contents.