GPIT subunits protein accumulation varies in different human cancers tissues as compared to corresponding normal tissues. Representative photomicrographs of sections of breast carcinoma, ovarian carcinoma, uterine carcinoma and their corresponding normal tissue sections immunostained with PIG-U, PIG-T, GPAA1, GPI8 and PIG-S antibodies and visualized by bright-field microscopy under ×100 magnification. All GPIT subunits, ie PIG-U, PIG-T, GPAA1, GPI8 and PIG-S, showed cytoplasmic localization. Overall, PIG-U, PIG-T, GPAA1, GPI8 and PIG-S proteins were uniformly overexpressed in tumor tissues as compared to normal.