Fig. 5.

Apigenin inhibits MCF-7 clonogenic survival. A, MCF-7 cells were treated with DMSO, 75 μM apigenin, or 75 μM baicalein for 3 days. Lysates were immunoblotted with the indicated antibodies. B, MCF-7 cells were treated with DMSO, 75 μM apigenin, or 75 μM baicalein for 3 days, stained with propidium iodide, and analyzed by flow cytometry. The percentage of cells with loss of cell membrane integrity is included. C, MCF-7 cells were plated at a density of 1000 cells/6-cm dish. At 24 h after seeding, DMSO or apigenin at concentrations of 25, 50, and 75 μM was added to the medium. After 2 weeks, colonies were stained with crystal violet. The results (mean ± S.D. of 3 determinations) are expressed as the percentage of control colony formation in the presence of DMSO.