Figure 3.

TCRB sequence diversity in the naïve and memory compartments of a deeply sequenced individual. (A) Frequency distributions of CD45RA+/CD45RO− (naïve) and CD45RA−/CD45RO+ (memory) T-cell subsets isolated by FACS from PBMCs from a separate blood sample from donor 1, taken on day 1. The similarity of the two curves reflects the presence of high diversity within both subsets, although this is slightly greater for the naïve subset, as evident from the extended tail of the distribution. There are, in both cases, a small number of extreme copy clonotypes and a relative clonotype abundance varying over four orders of magnitude. (B) There is more overlap with the deeply sequenced sorted cells for the memory versus the naïve subset, but even the overlap of the memory subset is modest. This is consistent with a large total repertoire size that can be only partially captured in a given blood draw. (C) Of the sequences that were shared between sorted naïve and memory cells on day 1, there was a preferential transition to the memory subset on day 8.