FIGURE 4.

Investigation of OR1740 receptor homo-oligomerization by BRET. A, the BRET level was measured either in disrupted yeast cells coexpressing OR1740-Rluc (donor) and OR1740-EYFP (acceptor) or in crude membranes prepared from these cells with 5 μm coelenterazine-h. The data are shown as the normalized BRET ratio. B, BRET assays were performed with clones with variable acceptor (OR1740-EYFP)/donor (OR1740-Rluc) ratios. BRET levels are plotted as a function of the acceptor/donor ratio, estimated from immunoblots as described under “Materials and Methods.” Data are representative of at least three independent experiments.