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Investigation of OR1740 receptor homo-oligomerization at the subcellular level by BRET. A, the BRET level was measured in each cellular membrane subfraction, obtained by sucrose gradient fractionation from crude membranes of yeast cells coexpressing OR1740-Rluc and OR1740-EYFP. B, all subfractions were analyzed by immunoblotting with antibodies targeting the yeast ER and plasma membrane markers Dpm1p (30 kDa) and Pma1 (100 kDa), respectively.
