TABLE 1.
Bacterial strains and plasmids
| Description | Source | |
|---|---|---|
| Strain | ||
| DSS301 | TG1, kanR ΔdmsABC | Lab collection (20) |
| TOPP2 | rifR (F′, proAB, lacIqZΔM15, Tn10 (tetR)) | Stratagene |
| Plasmid | ||
| pBR322 | TetR AmpR | Pharmacia |
| pDMS160 | dmsABC cloned into the EcoRI-SalI fragment of pBR322, AmpR | Lab collection (28) |
| R61K | pDMS160-dmsAR61KBC | This study |
| ΔN21 | pDMS160-dmsAΔN21BC | This study |
| CS1a | pDMS160-dmsACS1BC | This study |
| CS1 + R61K | pDMS160-dmsACS1+R61KBC | This study |
a For simplicity, this plasmid is named CS1. The actual mutations are V20Y, ΔN21, S24V, R25G, and P43G (Fig. 1).