FIGURE 1.

Mapping regions of LC/A that contribute to intracellular localization in Neuro-2A cells. Upper panel, pGFP-LC/A derivatives (inset, LC/A(K6A,K11A) is termed LC/A(DKM) were transfected into Nero-2A cell. After an overnight incubation, LC/A localization phenotypes were visualized using confocal microscope, and representative images are shown. Middle panel, cells were also lysed, and membrane and cytosolic fractions were generated by centrifugation and subjected to SDS-PAGE. LC/A was detected by Western blotting using anti-GFP antibody and quantified. Data are the average of three independent experiments. Error bars indicate S.D. Lower panel, the N terminus of LC/A was modeled in PyMOL, and surface lysine residues (Lys⁶ and Lys¹¹) were highlighted in blue. An alignment of the N termini of LC/A and LC/E is shown with surface-exposed residues that include Lys⁶ and Lys¹¹ highlighted.