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. 2011 Mar 4;286(17):15067–15072. doi: 10.1074/jbc.M111.224493

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Coexpression of SNAP-25(C85A,C88A,C90A,C92A) disrupts LC/A plasma membrane localization. Upper panel, Neuro-2A cells were transfected with pLC/A (blue) alone (SN-Endo) or with pSNAP-25 (SN-YFP) or pSNAP-25(C85A,C88A,C90A,C92A)-YFP (SN(4CM)). Cells were washed, fixed, permeabilized, and probed with α-SNAP-25 antibody (SN-Endo) and subjected to confocal microscopy alone or merged. Lower panel, greater than 100 cells were visually scored for cytosolic LC/A upon each transfection treatment. Numbers are the average of three independent experiments. Error bars indicate S.D. SN, SNAP-25.