FIGURE 7.

Cleavage of SNAP-25 by LC/A and LC/A(8–438). Two μm SNAP-25(1–206) was incubated with the indicated amounts of LC/A(8–425) (●) or LC/A (1–425) (■), or 2 μm SNAP-25(146–206) was incubated with the indicated amounts of LC/A(8–425) (▴) or LC/A (1–425) (▾) in 10 mm Tris-HCl and 20 mm NaCl for 15 min at 37 °C and stopped with sample buffer. The gel was stained for protein, and SNAP-25 cleavage was determined by densitometry. Stained gels shown are from a representative experiment analyzing 1.5 and 200 nm LCs. The percentage of substrate cleaved was plotted versus [LC]. Results are the average of at least three independent experiments. Error bars indicate S.D.