FIGURE 3.

Sequential processing of PCDH12 protein. Two different clones of CHO-PCDH12, called LC1 and CB2, were treated for 16 h with 5 μm lactacystin, 5 μm L685,458, and 10 μg/ml GM6001, either alone or in combination, as indicated. A, protein extracts were analyzed by Western blotting using anti-PCDH12 Cter antibody to visualize the full-length (FL) protein and the CTF, called CTF0, 1, and 2, that accumulate after inhibition of proteases. The fragment at 100 kDa (*), not regulated by pharmacological inhibitors, is an artifact of secondary antibody. The low abundance fragment at 38 kDa may represent a minor proteolytic event. B, scheme of PCDH12 proteolytic cleavage summarizing the above data.