FIGURE 4.

Induction of PCDH12 cleavage by phorbol esters. A, cell supernatant of CHO-PCDH12 was harvested after 24 h of treatment with PMA (0.1 μg/ml) and analyzed by Western blotting (WB, PCDH12 EC) together with that of noninduced cells (NI) and unconditioned medium (M). B, PCDH12 content in cell extracts of CHO-PCDH12 treated with PMA and harvested at indicated time. The cell extracts were analyzed by Western blotting using PCDH12 Cter and tubulin antibodies. C, three independent experiments were performed as in B. The signals were quantified and plotted as PCDH12: tubulin signal ratio versus time. The data represent the means ± S.D.