FIGURE 1.

Phenotypic analysis. A, 10-fold dilutions of exponentially growing yeast cells spotted onto glucose YPD or SD medium. Shown are carbonylation (B) and glutathionylation (C) levels in protein extracts as measured by chemiluminescence. 20 μg of total protein were loaded onto each lane in all cases. Representative sample immunoblots and quantitative analysis of three different experiments are shown. D, total levels of ROS in the cells. *, statistically significant (p < 0.05) relative to WT. Error bars, S.E.