TABLE 2.
Proteins with specifically modified cysteine(s) in the mutant strains
The abbreviated name of the proteins according to SGD or the ORF code are shown together with the position of the cysteine(s) residue(s) modified in the protein sequence. Criteria for inclusion of a protein in a particular group are explained under “Results” and in the legend to Fig. 2. *, the peptide detected does not contain cysteine. +, proteins detected only in the corresponding mutant (see also legend to Fig. 2); −, proteins are detected in all strains except in the corresponding mutant. Underlined names indicate that the pattern is the same in the double mutant.
| Grx2-specific |
Prx1-specific |
Doubly specific |
Synergic specific |
|||||
|---|---|---|---|---|---|---|---|---|
| Protein | Cys | Protein | Cys | Protein | Cys | Protein | Cys | |
| Biosynthesis | Aro4p (−) | 76, 244 | Aco1p (−) | 382 | Aah1p (+) | 142, 188, 252 | Erg2p (+) | 40 |
| Ham1p (+) | 123 | Asn2p (−) | 404, 431 | Adi1p (+) | 40 | Hom6p* (+) | ||
| Hem12p (+) | 26 | Bat1p (+) | 227, 361 | Adk1p (+) | 84 | Idi1p (−) | 58, 74 | |
| Lys9p (+) | 154, 340 | Fsh1p (−) | 39 | Bat2p (+) | 109 | |||
| Sam1p (+) | 91 | Gln1p (+) | 160 | Dys1p (+) | 208, 252 | |||
| Ura5p (+) | 86 | Lys4p (+) | 400/407/410 | Fcy1p (−) | 36, 71 | |||
| Ydl124wp (+) | 197 | Lys20p (−) | 202 | IIv3p (−) | 176, 258, 581 | |||
| Rib3p (−) | 34, 56, 133 | IIv5p (+) | 316 | |||||
| Trp5p (−) | 420 | IIv6p (+) | 270 | |||||
| Leu1p (+) | 63, 627 | |||||||
| Prs3p (+) | 273 | |||||||
| Transcription/Translation | Ala1p (+) | 532, 719 | Caf20p (−) | 82 | Pab1p (−) | 368 | Hyp2p* (−) | |
| Arc1p (−) | 266, 284 | Rnr2p (−) | 270 | Rpl18bp (−) | 121 | Rpl12bp (−) | 141 | |
| Rex2p (+) | 116 | Rpl23ap (+) | 25 | Rpl15bp (−) | 152 | Rps27ap* (−) | ||
| Rpc19p (+) | 138 | Rps17ap (−) | 35 | Rps0ap (−) | 162 | Ses1p (+) | 370, 400 | |
| Rps18bp (−) | 43, 113 | Rps1ap (−) | 69 | Vas1p (+) | 509, 758 | |||
| Rpl35ap (+) | 53 | Tif34p (−) | 67, 104 | |||||
| Sky1p (+) | 28 | Trm112p (+) | 16 | |||||
| Snu13p (+) | 91 | |||||||
| Tec1p (+) | 314 | |||||||
| Chaperone/Stress | Arf2p (+) | 159 | Dld3p (+) | 363 | Cct8p (+) | 252 | Cpr3p (−) | 137 |
| Ccs1p (+) | 159 | Dre2p (−) | 252 | Cpr5p (+) | 150 | Ykl069wp (−) | 91, 125 | |
| Hsp31p (+) | 138 | Hsp104p (+) | 209 | Sti1p (+) | 66 | |||
| Pdi1p (+) | 61/64, 406/409 | Ssb2p (−) | 20, 454 | |||||
| Yhb1p (+) | 328, 362 | |||||||
| Energy metabolism | Rpe1p (+) | 79 | Adh6p (−) | 101/103, 162 | Lsc1p (+) | 153 | Glo2p (−) | 18, 115, 127, 218 |
| Tkl1p (+) | 622 | Ald6p (+) | 133, 193/212 | Hrk1p (+) | 471 | |||
| Pgm2p* (−) | ||||||||
| Cell wall | Ccw14p (−) | 40/50/52 | Cwh41p (+) | 223 | ||||
| Guk1p (+) | 96 | Scw11p (+) | 323 | |||||
| Pmi40p (+) | 148, 291 | Mns1p (+) | 385 | |||||
| Psa1p (−) | 319 | Utr2p (+) | 67 | |||||
| Scw10p (+) | 176, 357 | |||||||
| Transport | Rad23p (+) | 37 | Prc1p (−) | 373/379, 409 | Pup2p (−) | 76, 221 | Doa1p (+) | 196 |
| Tfs1p (+) | 110/115 | Shp1p (−) | 88 | Ybr139wp (+) | 336 | |||
| Degradation/Proteases | Gre3p (+) | 20, 28 | Yrb1p (−) | 145 | Qcr2p (+) | 137 | Gda1p (+) | 87, 364 |
| Prd1p (+) | 478, 605 | Kin2p* (−) | ||||||
| Pep1p (+) | 896, 1096 | |||||||
| Other | Est2p* (+) | H+ATPase (−) | 19 | Acid phosphatase (−) | 276, 408/416 | Cin8p* (−) | ||
| Pho3p (−) | 263, 276, 408/416 | Pea2p* (−) | Pho84p (−) | 499 | Cpr1p (−) | 117 | ||
| Yor285wp (+) | 98 | Yar1p (+) | 44 | Pma1p (−) | 221, 409, 532 | Ddb1p (−) | 37/39, 87, 149 | |
| Ydl086wp (+) | 243 | Yjl217wp (−) | 170 | Pry2p (+) | 299 | Fas2p (+) | 1246 | |
| Yer079wp (−) | 59 | Ydr051cp (+) | 183 | Protoplast-secreted (−) | 80 | Hog1p (+) | 156 | |
| Ykl105cp (+) | 1023 | Ynl010wp (+) | 66 | Rhr2p (+) | 100 | orf 00954p (−) | 21/30/35/40 | |
| Ylr454wp* (+) | Unnamed* (−) | Yel047cp (+) | 196, 454 | Pyrophosphatase (+) | 83 | |||
| Ydr036p* (+) | Yir010wp* (+) | Yhr020wp (−) | 11 | |||||
| Yjr004cp (+) | 202 | Yjl218wp (−) | 178 | Ydr391cp (−) | 169 | |||
| Ygl137wp (−) | 160 | Pho8p (+) | 132, 150 | |||||
| Ydl037cp* (+) | ||||||||
| Yfr002wp* (−) | ||||||||