FIGURE 6.

Reduction of GSL levels by Genz-123346 inhibits Th₁₇ differentiation. Naive CD4⁺CD62L⁺ T cells from wild-type C57BL/6 mice were used to assess the effects of Genz-123346 on Th differentiation. A, effect of Genz-123346 on differentiation of wild-type naive T cells into Th₁ and Th₁₇ subsets. Intracellular cytokines were stained using fluorescently labeled antibodies. Plots depict the expression of IFN-γ and IL-17 in live CD4⁺ gated cell populations. B, effect of Genz-123346 on Th₁₇ differentiation as assessed by RORγt intracellular staining and IL-17 production. CTB denotes the GM1 level on the cell surface, which was reduced by Genz-123346 treatment. Results are representative of at least three studies.