FIGURE 7.

Reduction of GSL levels through inactivation of GM3S inhibits Th₁₇ differentiation. Naive CD4⁺CD62L⁺ T cells from GM3S KO (−/−) and heterozygous (+/−) mice and wild-type littermates (+/+) on a C57BL/6-129/svev mixed background were prepared and differentiated into various helper T cell subsets as described under “Experimental Procedures.” A, effect of GM3S deficiency on differentiation of naive T cells into Th₁ and Th₁₇ subsets. B, IL-17 levels in culture media of samples in A as measured by ELISA. Results are representative of at least three studies.