FIGURE 2.

Domain organization of the transcriptional corepressor MINT. MINT is a multidomain 3,664-residue protein composed of N- and C-terminal RNA recognition motifs (RRM) and Spen paralog and ortholog C-terminal domain (SPOC) domains, respectively. MINT also contains several nuclear localization sequences (NLS), a region that interacts with the transcription factor Msx2 (MSXB), and a CSL-interacting domain (CID). The constructs that were utilized in the herein described CSL-MINT binding study are expanded below the CSL-interacting domain.