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. 2011 Mar 3;286(17):14892–14902. doi: 10.1074/jbc.M110.181156

FIGURE 3.

FIGURE 3.

Secondary structure analysis of CSL, NICD, and MINT. The figure shows far-UV circular dichroism spectroscopy for MINT (2776–2833), the RAMANK domains of Notch, and CSL (RBP-J) in A, B, and C, respectively. Relative amounts of secondary-structure were determined from CD data using Dichroweb and CDSSTR (35, 36). The normalized root mean square deviation parameter values for analysis of the MINT, RAMANK, and CSL CD data are 0.02, 0.04, and 0.11, respectively. A, the CD spectrum of Mint (2776–2833) has a distinct minimum at 199 nm, characteristic of random coil. B, consistent with its largely helical structure and previously published results (48), the CD spectrum of RAMANK shows characteristic minima for α-helix at 207 and 222 nm. C, the CD spectrum of CSL displays a minimum at 215 nm, consistent with it being largely composed of β-sheet (41, 51). Error bars, S.D.