TABLE 1.
Calorimetric data for MINT binding to CSL
All experiments were performed at 25 °C. Values are the mean of at least three independent experiments, and errors represent the S.D. of multiple experiments.
| Macromolecule | MINT (ligand) | K | Kd | ΔG° | ΔH° | −TΔS° |
|---|---|---|---|---|---|---|
| m−1 | μm | kcal/mol | kcal/mol | kcal/mol | ||
| CSL | 2776–2833 | 9.4 ± 1.2 × 107 | 0.011 | −10.9 ± 0.1 | −14.6 ± 0.2 | 3.7 ± 0.2 |
| CSL + DNA | 2776–2833 | 7.3 ± 1.2 × 107 | 0.014 | −10.7 ± 0.1 | −14.8 ± 0.5 | 4.1 ± 0.6 |
| BTD | 2776–2833 | 2.5 ± 0.3 × 104 | 41 | −6.0 ± 0.1 | −14.9 ± 3.6 | 8.9 ± 3.5 |
| CTD | 2776–2833 | 1.7 ± 0.2 × 104 | 60 | −5.8 ± 0.1 | −7.7 ± 1.7 | 2.0 ± 1.9 |
| BTD-CTD | 2776–2833 | 1.0 ± 0.1 × 106 | 0.969 | −8.2 ± 0.1 | −14.2 ± 1.6 | 6.0 ± 1.7 |