TABLE 3.

Calorimetric data for SMT3-MINT fusion proteins binding to CSL

All experiments were performed at 25 °C. Values are the mean of at least three independent experiments, and errors represent the S.D. of multiple experiments.

Macromolecule	SMT3-MINT (ligand)	K	Kd	ΔG°	ΔH°	−TΔS°
		m−1	μm	kcal/mol	kcal/mol	kcal/mol
CSL	2776–2833	1.2 ± 0.02 × 108	0.008	−11.0 ± 0.01	−18.0 ± 0.6	7.0 ± 0.6
CSL	2776–2820	3.8 ± 1.1 × 108	0.003	−11.7 ± 0.2	−19.3 ± 0.5	7.6 ± 0.7
CSL	2776–2812	1.4 ± 0.5 × 108	0.008	−11.1 ± 0.2	−14.0 ± 1.1	2.9 ± 1.2
CSL	2791–2820	8.2 ± 1.8 × 104	13	−6.7 ± 0.1	−12.8 ± 1.9	6.1 ± 2.1
CSL	2791–2812	9.4 ± 1.3 × 104	11	−6.8 ± 0.1	−10.6 ± 1.3	3.8 ± 1.3
CSL	2752–2833a	1.9 ± 0.1 × 108	0.005	−11.3 ± 0.02	−14.8 ± 1.0	3.5 ± 1.0

^a The binding data reported for MINT (2752–2833) to CSL was done in the context of the free peptide and not the SMT3 fusion protein.