TABLE 4.

Temperature dependence of MINT, RAM, and RAMANK binding to CSL

Calorimetric data for the temperature dependence of MINT and RAM binding to CSL. For CSL-MINT and CSL-RAM binding experiments, CSL was placed in cells, and either MINT or RAM was placed in the syringe; however, for the CSL-RAMANK binding experiment, CSL and RAMANK were placed in the syringe and the cell, respectively. Values are the mean of at least three independent experiments, and the errors represent the S.D. of multiple experiments.

	Temperature	K	Kd	ΔG°	ΔH°	−TΔS°
	°C	m−1	μm	kcal/mol	kcal/mol	kcal/mol
CSL + MINT	5	6.2 ± 1.4 × 107	0.017	−9.9 ± 0.1	−3.9 ± 0.4	−6.0 ± 0.5
	15	8.3 ± 2.3 × 107	0.013	−10.4 ± 0.2	−6.3 ± 0.3	−4.2 ± 0.5
	25	9.4 ± 1.2 × 107	0.011	−10.9 ± 0.1	−14.6 ± 0.2	3.7 ± 0.2
	35	3.5 ± 0.6 × 107	0.029	−10.6 ± 0.1	−16.7 ± 0.7	6.1 ± 0.5
CSL + RAM	5	1.1 ± 0.5 × 108	0.012	−10.1 ± 0.3	−8.1 ± 0.4	−2.1 ± 0.4
	15	2.5 ± 0.7 × 108	0.004	−11.1 ± 0.2	−12.0 ± 0.1	0.9 ± 0.2
	25	6.8 ± 0.8 × 107	0.015	−10.7 ± 0.1	−18.2 ± 1.7	7.5 ± 1.6
	35	2.9 ± 0.4 × 107	0.035	−10.5 ± 0.1	−26.8 ± 3.5	16.3 ± 3.6
CSL + RAMANK	5	9.9 ± 4.4 × 108	0.001	−11.4 ± 0.2	−7.3 ± 1.4	−4.2 ± 1.5
	15	9.3 ± 1.6 × 107	0.011	−10.5 ± 0.1	−17.0 ± 0.1	6.5 ± 0.1
	25	7.2 ± 0.6 × 107	0.014	−10.7 ± 0.1	−17.7 ± 0.4	7.0 ± 0.3
	35	2.6 ± 0.2 × 107	0.039	−10.4 ± 0.1	−26.0 ± 1.0	15.6 ± 1.1