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The number of Arx+ cells is reduced in the Pdx1-Cre;Isl- 1LoxP/LoxP embryos. A–D, immunostaining with glucagon and Arx antibodies of E15.5 pancreatic sections from control and Pdx1-Cre;Isl-1LoxP/LoxP embryos. E, quantitative analysis of Arx+ cell populations as normalized to Pax6+ cells in the neighboring section. n = 6 for control and n = 5 for mutants. Data represents the mean ± S.E. *, p value <0.05.
