FIGURE 3.

Isl-1 binds to Re1 and Re2 of the mouse Arx gene. A, schematic diagram of the Arx locus illustrating the locations of Re1 (orange box, +5.6 to +6.1) and Re2 (green box, +23.6 to +24 kb). TSS, transcription start site; Ex, exon. B, the ChIP-Seq image depicts Isl-1 occupancy within Arx Re1 (chrX:89544959–89545500) and Re2 (chrX:89563002–89563440) in αTC1–6 cells. The conservation between mouse and other species within this region was shown using the University of California Santa Cruz genome browser. C and D, Re1 and Re2 binding to Isl-1 was readily detected in adult mouse islets and αTC1–6 cells by standard ChIP but to a lesser extent in βTC3 cells. Results were normalized to Isl-1 binding to the PEPCK promoter. Data are presented as the mean ± S.E. n = 3; p value <0.05.