FIGURE 5.

The Isl-1 binding sites in Re1 and Re2 are necessary for αTC1–6 cell activity. Sequence identity within Arx Re1 (A) and Arx Re2 (B) between mouse, human, and rat. Core Isl-1 binding site sequences are shown in bold, and the lines above demarcate the EMSA probe. Nucleotide mutated for luciferase reporter (C) and EMSA (Fig. 6C) assays are labeled in red. Nonconserved nucleotides are labeled in green. C, the activity of wild-type and Isl-1 binding site mutants (red) of pGL4.27-Re1 and pGL4.27-Re2 in αTC1–6 cells. The pRL-SV40 normalized data were presented as the mean ± S.E. *, p <0.05.