FIGURE 2.

Sodium phenylbutyrate protects N27 cells from oxidative stress and mutant α-synuclein toxicity. A–B and E–F, N27 cells were incubated with PB (0.15 mm) for 48 h, followed by 24 h treatment with H₂O₂ or 6-OHDA at various concentrations. Sample images were shown in A-B with cells identified by GFP adenovirus expression. Cell viability was determined by MTT assays and shown in E–F. C–D and G–H, N27 cells were incubated with various doses of PB for 48 h, followed by 48 h treatment of adenovirus expressing A53T human α-synuclein (200 pfu/cell). N27 cells with α-synuclein aggregates were identified by α-synuclein antibody LB509 staining, with sample images shown in C–D. Three random fields (150–200 cells per field) were examined to determine the percentage of cells with α-synuclein aggregates (arrows in G–H). Triplicate treatments in 24-well plates were used, and experiments were repeated three times. (*, p < 0.05; **, p < 0.01 compared with control, n = 9.)