FIGURE 3.
Knockdown of endogenous DJ-1 abolishes neuroprotection from sodium phenylbutyrate. The N27 cells were transduced with adenovirus expressing shDJ-1 or Ad-GFP at concentration of 200 pfu/cell for 2 days. The cells were then added with or without PB treatment for 48 h, followed by exposure to hydrogen peroxide (H2O2) or 6-hydroxydopamine (6-OHDA) for 24 h. A, sample Western blot showed more than 85% reduction of endogenous DJ-1 treated by shDJ-1 adenovirus. B and C, cell viability was measured by MTT assay. Results showed that knockdown of DJ-1 abolished neuroprotective effects of PB in N27 cells (compare vertical and horizontal bars to white or black bars). Triplicate treatments in 24-well plates were used, and experiments were repeated three times. (*, p < 0.05; **, p < 0.01 compared with control, n = 9.)