FIGURE 4.

Sodium phenylbutyrate and sodium butyrate increased DJ-1 expression in mouse brain. A–C, adult C57BL/6 mice (ages 4–6 months old) were treated with PB or SB at different doses in drinking water for 2 weeks. Brain tissues (cortex) were used for Western blotting with DJ-1, α-synuclein, and β-actin antibodies. Four mice were used for each dose, and an equal amount of protein was loaded in each lane after being normalized to β-actin. Typical Western blot images of DJ-1 and α-synuclein are shown in A. B–C, quantitative data of DJ-1 and α-synuclein levels after PB and SB treatment (*, p < 0.–05; **, p < 0.01 compared with control, n = 4.)